Lung Tissue Banks for Gene Expression Studies
نویسندگان
چکیده
منابع مشابه
CXCL10 Gene Expression in the Lung Tissue of Sulfur Mustard-Exposed Patients with Long-Term Pulmonary Complications
Aims: Mustard gas is an alkalizing substance that can lead to damage to DNA and intracellular enzymes. The aim of this study was to investigate the expression of CXCL10 in chemically-injured veterans’ lung tissue. Materials & Methods: This case-control study was done on 16 lung paraffin blocks of individuals exposed to mustard gas and 7 lungs paraffin blocks not-exposed people as...
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Epidemiological and mechanistic evidence on the association of quercetin-rich food intake with lung cancer risk and carcinogenesis are inconclusive. We investigated the role of dietary quercetin and the interaction between quercetin and P450 and glutathione S-transferase (GST) polymorphisms on lung cancer risk in 1822 incident lung cancer cases and 1991 frequency-matched controls from the Envir...
متن کاملExpression of the prostate specific antigen gene by lung tissue.
We describe a female patient with lung adenocarcinoma whose tumor extract was highly positive for prostate specific antigen (PSA) immunoreactivity. PSA was present in its Mr 33,000 free form. Using reverse transcription-PCR, we were able to amplify a 754-bp fragment that specifically hybridized to a PSA RNA probe on Southern blots. The PCR fragment was sequenced and found to represent PSA cDNA ...
متن کاملAssessment of Control Tissue for Gene and Protein Expression Studies: A Comparison of Three Alternative Lung Sources
The use of an appropriate control group in human research is essential in investigating the level of a pathological disorder. This study aimed to compare three alternative sources of control lung tissue and to determine their suitability for gene and protein expression studies. Gene and protein expression levels of the vascular endothelial growth factor (VEGF) and gelatinase families and their ...
متن کاملTRIzol-based RNA Extraction: A Reliable Method for Gene Expression Studies
RNA extraction is a prerequisite technique for gene expression studies, analyzing the etiology and disease progression, treatment effects, as well as designing the diagnostic methods. Although many RNA extraction kits have been commercialized, but these kits are expensive and are not accessible in some countries. Many studies have shown that TRIzol is an applicable material for the RNA extracti...
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ژورنال
عنوان ژورنال: COPD: Journal of Chronic Obstructive Pulmonary Disease
سال: 2004
ISSN: 1541-2555,1541-2563
DOI: 10.1081/copd-200032168